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Innoprot

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        • Enzyme Activity Determination Kits

pNPP Phosphatase Assay Kit

Reference: P40114


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Data Sheet

Protein phosphatase, enzyme that control the removal of phosphate (PO43-) group from protein molecules, regulates many fundamental cellular process such as cell attachment, proliferation, differentiation and apoptosis.


Assay Characteristics

Innoprot pNPP Phosphatase Assay is optimized to detect phosphatase activity in biological samples using pNPP (4-nitrophenyl phosphate) as a colorimetric substrate for most phosphatases. A water soluble yellow product with a strong absorption at 405 nm is developed during the reaction of pNPP with phosphatase and can be detected with an ELISA plate reader.


Kit Components

  • Reagents for 500 Tests in 96 well-plates

Reagent

Quantity

Storage

10X pNPP Stock

2.5 ml

- 20°C

Buffer-Acidic

25 ml

4°C

Buffer-Neutral

25 ml

4°C

Buffer-Alkaline

25 ml

4°C

Stop Buffer

25 ml

4°C

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