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Innoprot

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      • Classic Cell-based Assay Kits
        • Enzyme Activity Determination Kits

Superoxide Dismutase (SOD) Assay Kit

Reference: P40118


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Data Sheet

Superoxide dismutase (SOD), as one of the body’s most important defense mechanisms against free-radical damage, catalyzes the dismutation of the superoxide radical (O2-) into hydrogen peroxide (H2O2) and elemental oxygen (O2).


Assay Characteristics

Innoprot’s SOD Assay, the superoxide anions, generated from the conversion of xanthine to uric acid and hydrogen peroxide by xanthine oxidase (XOD), reduce WST-1 (4-[3-(4-iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate sodium salt) to water-soluble formazans, which can be measured by absorbance at 438 nm. SODs lower the rate of the reduction reaction by reducing superoxide anion concentrations. Therefore, the % Inhibition of the reduction reaction can be determined as a measurement of SOD activity.


Kit Components

  • Reagents for 100 tests

Reagent

Quantity

Storage

SOD Assay Buffer

25 ml

2 - 8°C

EDTA

5 ml

- 20°C

WST-1

5 ml

- 20°C

XOD

5 ml

- 20°C

Sodium Oxymate

5 ml

- 20°C

SOD Standard (80 U/ml) 0.5 ml - 20°C

Cell Lysis Buffer

10 ml

2 - 8°C

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