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Innoprot

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        • Enzyme Activity Determination Kits

Caspase 3 Activity Assay Kit

Reference: P40120


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Data Sheet

Caspase-3 is a member of caspases that plays a key role in mediating apoptosis, or programmed cell death. Upon activation, it cleaves a variety of cellular proteins, causing morphological and functional changes to cells undergoing apoptosis.


Assay Characteristics

The Innoprot Caspase-3 Activity Assay provides a quick and convenient method to measure caspase-3 activity. The colorimetric assay is based on the spectrophotometric detection of the chromophore p-nitroanilide (pNA) after its cleavage by caspases-3 from the labeled substrate acetyl-Asp-glu-Val-Asp p-nitroanilide (Ac-DEVD-pNA). The concentration of pNA is measured by absorbance at 405 nm. The caspase-3 activity can be calculated as µmol of pNA released per minute per milliliter of cell lysate. 


Kit Components

  • Reagents for 100 Tests

Reagent

Quantity

Storage

Lysis Buffer

10 ml

2 - 8°C

DTT Stock (1 M)

0.2 ml

- 20°C

Assay Buffer 1X

3 ml

2 - 8°C

Caspase 3 Substrate (2mM)

1 ml

- 20°C, dark

pNA Standard (40 mM)

20 ul

- 20°C

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