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Innoprot

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HiTSeeker CRHR2 (Corticotropin Releasing Hormone Receptor 2)

Reference: P30116


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Data Sheet

Each vial of HiTSeeker CRHR2 contains HEK293 cells stably expressing human Corticotropin Releasing Hormone Receptor 2 (CRHR2) with no tag. We offer this cell line as a “stable cell line” but we are now also offering vials of division-arrested cells (DA cells) in order to perform a small number of assays. Each vial of these DA cells contains 2.5 million cells, an enough number of cells to perform a complete experiment in a 96 well-plate or 384 well-plate.

Innoprot HiTSeeker CRHR2 Assay Cell Line has been designed to assay compounds or analyze their capability to modulate Corticotropin Releasing Hormone Receptor 2. When CRF binds to the CRHR2 a G protein is activated, which in turn, triggers a cellular response mediated by second messengers (Calcium and cAMP). This cell line has been validated measuring calcium and cAMP increase in the cytosol. The high reproducibility of this assay allows monitoring CRHR2 receptor activation process in High Throughput Screening.


Materials Provided


  • P30116: 2 vials of 3 million stably transfected HEK293 Cells expressing CRHR2
  • P30116-DA: 1 vial with 2.5 million Division Arrested HEK293 Cells expressing CRHR2 (READY TO USE)
 

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