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Innoprot

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        • GPCR CELL LINES

HiTSeeker GRPR (Gastrin Releasing Peptide Receptor)

Reference: P30150


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Data Sheet

Each vial of HiTSeeker GRPR Assay Cell Line contains U2OS cells stably expressing human Gastrin Releasing Peptide Receptor (GRPR) with no tag. We offer this cell line as a “stable cell line” but we are now also offering vials of division-arrested cells (DA cells) in order to perform a small number of assays. Each vial of these DA cells contains 2.5 million cells, an enough number of cells to perform a complete experiment in a 96 well-plate or 384 well-plate.

Innoprot HiTSeeker GRPR Assay Cell Line has been designed to assay compounds or analyze their capability to modulate Gastrin Releasing Peptide Receptor. When Gastrin Releasing Peptide (GRP) binds to the GRPR a G protein is activated, which in turn, triggers a cellular response mediated by second messengers (e.g. Calcium). This cell line has been validated measuring calcium increase in the cytosol. The high reproducibility of this assay allows monitoring GRP Receptor activation process in High Throughput Screening.


Materials Provided


  • P30150: 2 vials of 3 million stably transfected U2OS Cells expressing GRPR
  • P30150-DA: 1 vial with 2.5 million Division Arrested U2OS Cells expressing GRPR (READY TO USE)
 

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