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  • Innoprot
    • Productos
      • GPCR Cell Lines

HiTSeeker HTR2A (5-Hydroxytryptamine Receptor 2A)

Reference: P30160

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Data Sheet

Each vial of HiTSeeker HTR2A (5-HT2A) contains U2OS cells stably expressing Human 5-Hydroxytryptamine Receptor 2A with no tag. We offer this cell line as a “stable cell line” but we are now also offering vials of division-arrested cells (DA cells) in order to perform a small number of assays. Each vial of these DA cells contains 2.5 million cells, an enough number of cells to perform a complete experiment in a 96 well-plate or 384 well-plate.

Innoprot HiTSeeker 5-HT2A Cell Line has been designed to assay compounds or analyze their capability to modulate 5-Hydroxytryptamine Receptor 2A. When 5-HT binds to the 5-HT2A a G protein is activated, which in turn, triggers a cellular response mediated by second messengers (Ca). This cell line has been validated measuring Calcium increase in the cytosol. The high reproducibility of this assay allows monitoring 5-Hydroxytryptamine Receptor 2A  activation process in High Throughput Screening.

Materials Provided

  • P30160: 2 vials of 3 million stably transfected U2OS Cells expressing 5-HT2A
  • P30160-DA: 1 vial with 2.5 million Division Arrested U2OS Cells expressing 5-HT2A (READY TO USE)

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