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Innoprot

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HiTSeeker VIPR1 (Vasoactive Intestinal Polypeptide Receptor 1)

Reference: P30112


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Data Sheet

Each vial of HiTSeeker VIPR1 contains HEK293 cells stably expressing human Vasoactive Intestinal Polypeptide Receptor 1 with no tag. We offer this cell line as a “stable cell line” but we are now also offering vials of division-arrested cells (DA cells) in order to perform a small number of assays. Each vial of these DA cells contains 2.5 million cells, an enough number of cells to perform a complete experiment in a 96 well-plate or 384 well-plate.

Innoprot's HiTSeeker VIPR1 Cell Line has been designed to assay compounds or analyze their capability to modulate human Vasoactive Intestinal Polypeptide Receptor 1. When an agonist (like PACAP-38) binds to the VIPR1 a G protein is activated, which in turn, triggers a cellular response mediated by second messengers (cAMP). This cell line has been validated measuring cAMP increase in the cytosol. The high reproducibility of this assay allows monitoring Vasoactive Intestinal Polypeptide Receptor 1 activation process in High Throughput Screening.


Materials Provided


  • P30112: 2 vials of 3 million stably transfected HEK293 Cells expressing VIPR1
  • P30112-DA: 1 vial with 2.5 million Division Arrested HEK293 Cells expressing VIPR1 (READY TO USE)
 

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