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Innoprot

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        • GPCR CELL LINES

HiTSeeker TSHR (Thyroid Stimulating Hormone Receptor)

Reference: P30199


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Data Sheet

Each vial of HiTSeeker TSHR contains HEK293 cells stably expressing human Thyroid Stimulating Hormone Receptor with no tag. We offer this cell line as a “stable cell line” but we are now also offering vials of division-arrested cells (DA cells) in order to perform a small number of assays. Each vial of these DA cells contains 2.5 million cells, an enough number of cells to perform a complete experiment in a 96 well-plate or 384 well-plate.

Innoprot's HiTSeeker TSHR Cell Line has been designed to assay compounds or analyze their capability to modulate human Thyroid Stimulating Hormone Receptor. When an agonist (like TSH) binds to the TSHR a G protein is activated, which in turn, triggers a cellular response mediated by second messengers (cAMP). This cell line has been validated measuring cAMP increase in the cytosol. The high reproducibility of this assay allows monitoring Thyroid Stimulating Hormone Receptor activation process in High Throughput Screening.


Materials Provided


  • P30199: 2 vials of 3 million stably transfected HEK293 Cells expressing TSHR
  • P30199-DA: 1 vial with 2.5 million Division Arrested HEK293 Cells expressing TSHR (READY TO USE)
 

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