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Innoprot

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        • GPCR CELL LINES

HiTSeeker MC4R (Melanocortin 4 Receptor)

Reference: P30410


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Data Sheet

Each vial of HiTSeeker MC4R contains HEK293 cells stably expressing human Melanocortin 4 Receptor (with no tag. We offer this cell line as a “stable cell line” but we are now also offering vials of division-arrested cells (DA cells) in order to perform a small number of assays. Each vial of these DA cells contains 2.5 million cells, an enough number of cells to perform a complete experiment in a 96 well-plate or 384 well-plate.

Innoprot HiTSeeker MC4R Cell Line has been designed to assay compounds or analyze their capability to modulate human Melanocortin 4 Receptor. When an agonist (like a-MSH) binds to the MC4R a G protein is activated, which in turn, triggers a cellular response mediated by second messengers (cAMP). This cell line has been validated measuring cAMP increase in the cytosol. The high reproducibility of this assay allows monitoring Melanocortin 4 Receptor activation process in High Throughput Screening.


Materials Provided


  • P30410: 2 vials of 3 million stably transfected HEK293 Cells expressing MC4R
  • P30410-DA: 1 vial with 2.5 million Division Arrested HEK293 Cells expressing MC4R (READY TO USE)
 

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