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  • Innoprot
    • Productos
      • GPCR Cell Lines

HiTSeeker PTGER4 (Prostaglandin E Receptor 4)

Reference: P30405

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Data Sheet

Each vial of HiTSeeker PTGER4 contains HEK293 cells stably expressing human Prostaglandin E Receptor 4 (subtype EP4) with no tag. We offer this cell line as a “stable cell line” but we are now also offering vials of division-arrested cells (DA cells) in order to perform a small number of assays. Each vial of these DA cells contains 2.5 million cells, an enough number of cells to perform a complete experiment in a 96 well-plate or 384 well-plate.

Innoprot HiTSeeker PTGER4 Cell Line has been designed to assay compounds or analyze their capability to modulate human Prostaglandin E Receptor 4 - subtype EP4. When an agonist (like Prostaglandin E2) binds to the PTGER4 a G protein is activated, which in turn, triggers a cellular response mediated by second messengers (cAMP). This cell line has been validated measuring cAMP increase in the cytosol. The high reproducibility of this assay allows monitoring Prostaglandin E Receptor 4 - subtype EP4 activation process in High Throughput Screening.

Materials Provided

  • P30405: 2 vials of 3 million stably transfected HEK293 Cells expressing PTGER4
  • P30405-DA: 1 vial with 2.5 million Division Arrested HEK293 Cells expressing PTGER4 (READY TO USE)

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