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  • Innoprot
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      • GPCR Cell Lines

HiTSeeker TRH1 (Thyrotropin releasing hormone receptor)

Reference: P30423

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Data Sheet

Each vial of HiTSeeker TRH1 contains U2OS cells stably expressing human Thyrotropin-releasing hormone receptor with no tag. We offer this cell line as a “stable cell line” but we are now also offering vials of division-arrested cells (DA cells) in order to perform a small number of assays. Each vial of these DA cells contains 2.5 million cells, an enough number of cells to perform a complete experiment in a 96 well-plate or 384 well-plate.

Innoprot HiTSeeker TRH1 Cell Line has been designed to assay compounds or analyze their capability to modulate human Thyrotropin-releasing hormone receptor. When an agonist (like Thyrotropin‐releasing hormone) binds to the TRH1 a G protein is activated, which in turn, triggers a cellular response mediated by second messengers (Ca). This cell line has been validated measuring Calcium increase in the cytosol. The high reproducibility of this assay allows monitoring Thyrotropin‐releasing hormone receptor activation process in High Throughput Screening.

Materials Provided

  • P30423: 2 vials of 3 million stably transfected U2OS Cells expressing TRH1
  • P30423-DA: 1 vial with 2.5 million Division Arrested U2OS Cells expressing TRH1 (READY TO USE)

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