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      • GPCR Cell Lines

FluoHiTSeeker LHCGR (Luteinizing Hormone - Choriogonadotropin Receptor)

Reference: P30177-F

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Data Sheet

Each vial of FluoHiTSeeker LHCGR Assay contains 3 million of HEK293 cells stably co-expressing human Luteinizing Hormone/Choriogonadotropin Receptor (LHCGR) with no tag and the CRE-tGFP reporter gene. We offer this cell line as a “stable cell line” but we are now also offering vials of division-arrested cells (DA cells) in order to perform a small number of assays. Each vial of these DA cells contains 2.5 million cells, an enough number of cells to perform a complete experiment in a 96 well-plate or 384 well-plate.

Innoprot FluoHiTSeeker LHCGR Cell Line has been designed to assay compounds or analyze their capability to modulate Luteinizing Hormone/Choriogonadotropin Receptor. When an agonist binds to the LHCGR a G protein is activated, which in turn, triggers a cellular response mediated by cAMP. Due to the presence of the CRE-tGFP reporter gene, this cell line allows to monitor cAMP/PKA signaling pathway activity in cells. FluoHiTSeeker LHCGR cell line has been validated measuring cAMP increase in the cytosol through fluorescence intensity readout. The high reproducibility of this assay allows monitoring LHCG receptor activation process in High Throughput Screening.

Materials Provided

  • P30177-F: 2 vials of 3 million stably transfected HEK293 Cells expressing LHCGR & CRE-tGFP
  • P30177F-DA: 1 vial with 2.5 million Division Arrested HEK293 Cells expressing LHCGR & CRE-TGFP (READY TO USE)

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