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Innoprot

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FluoHiTSeeker FSHR (Follicle Stimulating Hormone Receptor)

Reference: P30117-F


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Data Sheet

Each vial of FluoHiTSeeker FSHR Assay contains 3 million of HEK293 cells stably co-expressing human Follicle Stimulating Hormone Receptor (FSHR) with no tag and the CRE-tGFP reporter gene. We offer this cell line as a “stable cell line” but we are now also offering vials of division-arrested cells (DA cells) in order to perform a small number of assays. Each vial of these DA cells contains 2.5 million cells, an enough number of cells to perform a complete experiment in a 96 well-plate or 384 well-plate.

Innoprot FluoHiTSeeker FSHR Cell Line has been designed to assay compounds or analyze their capability to modulate Follicle Stimulating Hormone Receptor. When an agonist binds to the FSHR a G protein is activated, which in turn, triggers a cellular response mediated by cAMP. Due to the presence of the CRE-tGFP reporter gene, this cell line allows to monitor cAMP/PKA signaling pathway activity in cells. FluoHiTSeeker FSHR cell line has been validated measuring cAMP increase in the cytosol through fluorescence intensity readout. The high reproducibility of this assay allows monitoring FSH receptor activation process in High Throughput Screening.


Materials Provided


  • P30117-F: 2 vials of 3 million stably transfected HEK293 Cells expressing FSHR & CRE-tGFP
  • P30117F-DA: 1 vial with 2.5 million Division Arrested HEK293 Cells expressing FSHR & CRE-TGFP (READY TO USE)
 

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