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Innoprot

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      • Classic Cell-based Assay Kits
        • Kits for Cell Analysis

WST 1 Cell Viability & Proliferation Assay Kit

Reference: P40102


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Data Sheet

The study of cell viability and proliferation is very important for evaluating a cell population''''''''s responses to external factors, such as growth factors, antibiotics and anti-cancer drugs.

Assay Characteristics


The reduction of tetrazolim salts to colored formazan compounds by succinate-tetrazolim reductase in live cells provides a sensitive and accurate method to measure cell viability and proliferation. The most commonly used tetrazolim salt, MTT [3-(4,5- Dimethylthiazol- 2-yl)-2,5 -diphenyltetrazolium bromide], however, suffers from the disadvantage that the formazan dye produced from MTT is extremely water insoluble, so an additional extraction step is needed for spectrophotometric quantification. Instead of MTT, the Innoprot WST-1 Cell Viability & Proliferation Assay utilizes a tetrazolim salt WST-1[2- (4- Iodophenyl)-3 -(4-nitrophenyl) -5- (2,4-disulfophenyl)-2H-tetrazolium]. WST-1 produces a highly water soluble formazan upon metabolically active cells, allowing a direct and user-friendly colorimetric measurement of cell viability and proliferation.

Kit Components

  • Reagents for 1000 Tests in 96-Well Microplates

Reagent

Quantity

Storage

WST 1 powder

5 x 6.52 mg

-20°C

Electro Coupling Reagent

5 x 2 ml

-20°C

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