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Cell Senescence Assay Kit

Reference: P40103


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Data Sheet

Normal mammalian cells divide for a limited number of population doublings and eventually enter an arrested state in which the cells remain alive, but do not proliferate in response to mitogens, and assume a characteristic enlarged, flattened morphology. This process, termed senescence, is thought to be a tumor suppressive mechanism and underlying cause of aging. Senescence-associated beta-galactosidase (SA- beta-gal) is a widely used biochemical maker for assessing senescence in cultured cells.

Assay Characteristics


Innoprot Cell Senescence Assay provides an easy-to-use method to detect SA- beta-gal by staining cells with 5-bromo-4-chloro-3-indolyl- beta-D-galactopyranoside (X-gal) at pH 6.0, a pH condition that suppress lysosomal beta-galactosidase activity sufficiently to ensure that nonsenescent cells remain unstained.

Kit Components

  • Reagents for 50 Tests in 33 mm plates

Reagent

Quantity

Storage

Staining Solution A

1 x 1 ml

4°C

Staining Solution B

1 x 1 ml

4°C

Staining Solution C
1 x 0,2 ml 4°C
Staining Solution D
1 x 20 ml 4°C
X-Gal Solution
1 x 5 ml -20°C
100X Fixing Solution
1 x 1 ml 4°C

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