X

This site uses third parties to own and optimize your navigation and perform analytical work cookies. If you continue browsing, consider that you accept its use.
You can learn more at our Cookie Policy.

Innoprot

  • Innoprot
    • Productos
      • Classic Cell-based Assay Kits
        • Kits for Cell Analysis

LDH Cytotoxicity Assay Kit

Reference: P40104


Get your Price

Data Sheet

Lactate dehydrogenase (LDH), which is a soluble cytosolic enzyme present in most eukaryotic cells, releases into culture medium upon cell death due to damage of plasma membrane. 


Assay Characteristics

The increase of the LDH activity in culture supernatant is proportional to the number of lysed cells. Innoprot LDH Cytotoxicity Assay kit provides a colorimetric method to measure LDH activity using a reaction cocktails containing lactate, NAD+, diaphrose and INT. LDH catalyses the reduction of NAD+ to NADH in the presence of L-lactate, while the formation of NADH can be measured in a coupled reaction in which the tetrazolium salt INT is reduced to a red formazan product. The amount of the highly colored and soluble formazan can be measured at 490 nm spectrophotometrically.


Kit Components

  • Reagents for 500 Tests in 96 well-plates

Reagent

Quantity

Storage

LDH Standard

1 ml

4°C

Sodium Lactate, 10X

1 ml

4°C

INT, 10X

1 ml

- 20°C

Substrate Mix

10 ml

- 20°C

Sodium Oxymate

10 ml

4°C

LDH Standard

1 ml

4°C

Related Products/Services



© Innoprot 2008 - Cell Based Assays for Drug Discovery Screening - Cookie Policy