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Innoprot

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TUNEL Apoptosis Assay Kit

Reference: P40106


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Data Sheet

Assay Characteristics


Innoprot Colorimetric TUNEL Apoptosis Assay is used for detection of apoptosis (programmed cell death) in individual cells based on the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick-end-labeling (TUNEL) technology. In brief, the 3''-OH end of the DNA strand breaks in apoptotic cells is labeled with biotinylated nucleotides using the enzyme TdT. Streptavidin conjugated horseradish peroxidase (HRP) is then bound to the biotinylated nucleotides, and visualized using the peroxidase substrate, 3,3''''''''''''''''-diaminobenzidine (DAB). The nuclei of apoptotic cells should be observed dark brown under light microscope.


Kit Components

  • Reagents for 50 Test in 96-Wells Plates

Reagents

Quantity

Storage

ECM Gel Solution

5 ml

-20°C

Solution TdT 250 ml -20°C
Solution Biotin-dUTP 2.5 ml -20°C
Streptavidin-HRP 50 ml 4°C, dark

Stock DAB-Sustrate

250 ml

2 - 8 °C

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