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in vitro Tube Formation Assay Kit (Angiogenesis Assay)

Reference: P40111

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Data Sheet

Angiogenesis, or the formation of new blood vessels, plays an important role in many physiological (e.g. wound healing) and pathological (e.g. tumor growth and inflammation disease) processes. As a complex multistep process, angiogenesis involves degradation of basement membrane,proliferation and migration of endothelial cells into extracellular matrix (ECM), followed by alignment and reorganization of endothelial cells into three-dimensional tubular structure and formation of new basement membrane. Specifically, the critical step of the formation of three-dimensional tubular structure by endothelial cells is the objective of many in vivo and in vitro angiogenesis assays to screen efficacy of both pro- and antiangiogenic agents.

Assay Principle

Innoprot’s In Vitro Tube Formation Assay provides a convenient system to measure the formation of 3D tubular structure by endothelial cells when cultured on an ECM gel of basement membrane extract. The ECM gel, which is prepared from Engelbreth Holm-Swarm (EHS) sarcoma produced in mice, consists of laminin, collagen IV, heparan sulfate proteoglycan, entactin and other minor components optimized for tube formation. With this system, tube formation can be achieved within 6-18 hours, with the earliest signs of tubular structure shown up after 3-4 hours. The Assay also provides a colorimetric cell staining reagent for better examination

Kit Components

  • Reagents for 50 Test in 96-Wells Plates




ECM Gel Solution

2.5 ml


Staining Solution B

2.5 ml

2 - 8 °C

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