X

This site uses third parties to own and optimize your navigation and perform analytical work cookies. If you continue browsing, consider that you accept its use.
You can learn more at our Cookie Policy.

Innoprot

  • Innoprot
    • Productos
      • Classic Cell-based Assay Kits
        • Kits for Cell Analysis

in vitro Tube Formation Assay Kit (Angiogenesis Assay)

Reference: P40111


Get your Price

Data Sheet

Angiogenesis, or the formation of new blood vessels, plays an important role in many physiological (e.g. wound healing) and pathological (e.g. tumor growth and inflammation disease) processes. As a complex multistep process, angiogenesis involves degradation of basement membrane,proliferation and migration of endothelial cells into extracellular matrix (ECM), followed by alignment and reorganization of endothelial cells into three-dimensional tubular structure and formation of new basement membrane. Specifically, the critical step of the formation of three-dimensional tubular structure by endothelial cells is the objective of many in vivo and in vitro angiogenesis assays to screen efficacy of both pro- and antiangiogenic agents.

Assay Principle


Innoprot’s In Vitro Tube Formation Assay provides a convenient system to measure the formation of 3D tubular structure by endothelial cells when cultured on an ECM gel of basement membrane extract. The ECM gel, which is prepared from Engelbreth Holm-Swarm (EHS) sarcoma produced in mice, consists of laminin, collagen IV, heparan sulfate proteoglycan, entactin and other minor components optimized for tube formation. With this system, tube formation can be achieved within 6-18 hours, with the earliest signs of tubular structure shown up after 3-4 hours. The Assay also provides a colorimetric cell staining reagent for better examination


Kit Components

  • Reagents for 50 Test in 96-Wells Plates

Reagents

Quantity

Storage

ECM Gel Solution

2.5 ml

-20°C

Staining Solution B

2.5 ml

2 - 8 °C

Related Products/Services



© Innoprot 2008 - Cell Based Assays for Drug Discovery Screening - Cookie Policy