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Innoprot

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Mycoplasma PCR Detection Kit

Reference: P40121


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Data Sheet

Assay Characteristics


Innoprot Mycoplasma PCR Detection Kit is a 16S rRNA-based PCR assay including 2× reaction buffer, primer set, as well as internal amplification control (IAC) and positive sample control. The genus-specific primer set we select recognizes the five typical contaminating mycoplasma species (i.e. M. hyorhinis, M. arginini, M. orale, M. fermentans, and A. laidlawi), which account for 98% of contaminations, as well as members of the genera Ureaplasma, Spiroplasma, and Acholeplasma, which account for the remaining 2% of contaminations. Eukaryotic and bacterial DNA is not amplified by this kit. The IAC is designed to be co-amplified simultaneously with the target mycoplasma sequence by the same set of primers. The resulted control band, which is distinguished from the target band by a difference in molecular mass, indicates the occurrence of DNA amplification. Therefore, false negative results due to the presence of PCR inhibitors in some cell cultures can be identified. Our kit also provides a positive sample control, which is noninfectious genomic DNA (gDNA) of M. fermentans. On the other hand, a no template (i.e. DNA-free DI H2O) negative sample control can help to determine whether there is a false positive result due to carry-over contamination. Each experiment should include both positive and negative sample controls, as well as the IAC.


Kit Components

  • Reagents for 100 Tests

Reagents

Quantity

Storage

2 x PCR Buffer

1.25 ml

-20°C

Mycoplasma primer 100 ul -20°C
IAC 100 ul -20°C
M. fermentans positive sample control 20 ul -20º C

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