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Innoprot

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Calcium Colorimetric Assay Kit

Reference: P40108


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Data Sheet

Introduction


As an essential mineral for all living organisms, 99% of calcium is deposited in bones and teeth, while 1% is found in extracellular fluid or within cells bound to proteins. Changes in the level of calcium in body fluid, such as serum, are found to be associated with many diseases such as hyperparathyroidism, neoplastic diseases, hypoparathyroidism, nephrosis, etc.


Assay Principle


Innoprot provides a simple and direct colorimetric measurement of calcium concentration in biological samples, utilizing the purple-red complex formed between calcium and ortho-cresolphthalein in alkaline medium. The optimized formulation eliminates the interference from magnesium and helps to release calcium bound to proteins. Intensity of the developed color (lambda=570 nm) is proportional to the calcium concentration in the physiologically important range (0.3-20 mM).

Kit Contents

  • Reagents for 250 Tests

Reagents

Quantity

Storage

Calcium Standard 20mM

1 ml

4°C

AMP Buffer

50 ml

4°C, Dark

Color Reagent
50 ml 4°C, Dark

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