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Innoprot

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      • Classic Cell-based Assay Kits
        • Oxidative Stress

Nitric Oxide Assay Kit

Reference: P40113


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Data Sheet

Nitric Oxide (NO), produced endogenously from L-Arginine by nitric oxide synthetases, plays an important role in many physiological processes including vascular regulation, immune responses, and neural communication. NO is extremely unstable and undergoes repaid oxidative degradation to nitrite (NO2-) and nitrate (NO3-), which can be spectrophotometrically determined.


Assay Characteristics


Innoprot’s Colorimetric Nitric Oxide Assay kit provides an accurate measurement of NO level in a simple two-step process: the reduction of nitrate to nitrite by vanadium (III) chloride, followed by quantification of nitrite by Griess reaction.


Kit Components

  • Reagents for 250 Tests

Reagent

Quantity

Storage

Nitrate Standard  200 μM

2.5 ml

4°C, dark

Vanadium Chloride

2.5 ml

4°C, dark

Griess Reagent I
12.5 ml 4°C, dark
Griess Reagent II 12.5 ml 4°C, dark
20 x ZnSO4 1.25 ml 4°C

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