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Innoprot

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      • Classic Cell-based Assay Kits
        • Oxidative Stress

Total Antioxidant Capacity (TAC) Assay Kit

Reference: P40117


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Data Sheet

Antioxidants are a group of free radical scavengers, including macro and micro molecules (transferrin, vitamin E, etc.) and enzymes (catalase, GSH reductase, etc.), that can protect vital cellular target against oxidative attack from free radicals or reactive oxygen species (ROS). Due to the chemical diversity and cooperative nature of antioxidants, the measurement of total antioxidant capacity (TAC) is important in monitoring the clinical status of body fluids, as well as the cumulative capability of various food supplements to counteract oxidative stress.


Assay Characteristics

The Innoprot Total Antioxidant Capacity (TAC) Assay is a cupric ion reducing antioxidant capacity (CUPRAC) spectrophotometric method, which can simultaneously measure hydrophilic and lipophilic antioxidants at physiological pH. Trolox® a cell-permeable, water-soluble derivative of vitamin E with antioxidant properties, serves as a standard.


Kit Components

  • Reagents for 100 Tests in 96 well-plates

Reagent

Quantity

Storage

Reagent CUPRAC

30 ml

2 - 8°C

TAC Buffer Assay

12.5 ml

2 - 8°C

Trolox Standard (0.5 mM)

2.5 ml

- 20°C

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