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      • Whole Fluorescent Cell Lines
        • TTFLUOR Primary Cells

TTFLUOR primary HUVEC - tube formation assay cells

Reference: P20201


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Data Sheet

TTFFLUOR HUVEC are Green fluorescent primary HUVEC which has been developed through transient transfection with tGFP expresion vector expressing the green fluorescent protein gene sequences as free cytoplasmatic protein.

TTFLUOR HUVEC are intended to be used in cell-based assay applications providing consistent levels of tGFP expression during two passages, which helps to simplify the interpretation of results. Endothelial cells processes as tube formation, can be study in vitro using TTFLUOR HUVEC following our standardized protocols.

TTFLUOR HUVEC cells have been assayed for tube formation with increasing concentrations of suramin and quantified using the ATTOVISION software. Parameters analyzed have been length of tubes, number of tubes and average of tube length obtaining data which fitted to a sigmoid-4-parameter logistic model for IC50 calculation.


Format

  • Crypreserved vial of 3 x106 cells for assay in 96-well plate
 

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