This site uses third parties to own and optimize your navigation and perform analytical work cookies. If you continue browsing, consider that you accept its use.
You can learn more at our Cookie Policy.


  • Innoprot
    • Productos
      • GPCR Cell Lines
        • cAMP-NOMAD GPCR Cell Lines

VIPR1 Nomad Cell Line

Reference: P70201

Get your Price

Data Sheet

A novel VIPR1 Nomad cell line has been developed stably transfecting cAMP Nomad biosensor and human vasoactive intestinal peptide (VIP) receptor 1 termed VPAC1 into U2OS cell line.

Innoprot cAMP Nomad VIPR1 cell line has been designed to assay compounds or analyze their capability to modulate vasoactive intestinal peptide receptor 1. When the agonist binds to VIPR1 a G protein is activated, which in turn, triggers a cellular response mediated by second messengers (cAMP).

Assay Details

In absence of cAMP, the fluorescent biosensor is localized in the cellular membrane. An increase in this second messenger concentration leads to change in the structural folding of Nomad Biosensor that promotes its cellular relocation in the vesicular trafficking of the cells. Activity is easily quantified on living cells by fluorescence intensity in any microplate reader.

This highly reproducible assay has been validated using PACAP-38 as a VIPR1 agonist in a High Throughput Screening (HTS) Platform. This cell line has been validated measuring cAMP increase in the cytosol analyzing Nomad biosensor distribution within the cell and by fluorescence intensity, showing an average of Z´= 0.80 +/- 0.01 for High Throughput Screening.

Materials Provided

  • P70201: 2 vials of 3 million stably transfected U2OS Cells expressing VIP receptor 1 and Nomad Biosensor
  • P70201-DA: 1 vial with 2 million Division Arrested U2OS Cells expressing VIP receptor 1 and Nomad Biosensor (READY TO USE)

Related Products/Services


© Innoprot 2008 - Cell Based Assays for Drug Discovery Screening - Cookie Policy