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        • cAMP-NOMAD GPCR Cell Lines

MCR3 Nomad Cell Line

Reference: P70204


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Data Sheet

A novel MCR3 Nomad cell line has been developed stably transfecting cAMP Nomad biosensor and Melanocortin Receptor 3 termed MCR3 into U2OS cell line.

Innoprot cAMP Nomad MCR3 cell line has been designed to assay compounds or analyze their capability to modulate Melanocortin Receptor 3. When the agonist binds to MCR3 a G protein is activated, which in turn, triggers a cellular response mediated by second messengers (cAMP).


Assay Details


In absence of cAMP, the fluorescent biosensor is localized in the cellular membrane. An increase in this second messenger concentration leads to change in the structural folding of Nomad Biosensor that promotes its cellular relocation in the vesicular trafficking of the cells. Activity is easily quantified on living cells by image analysis of cytoplasmatic granularity changes.

This highly reproducible assay has been validated using (Nle4,D-Phe7)-amelanocyte-stimulating hormone as a MCR3 agonist in a High Content Analysis (HCA) Platform. This cell line has been validated measuring cAMP increase in the cytosol analyzing Nomad biosensor distribution within the cell, showing an average of Z´= 0.83 +/- 0.01 for High Content Screening.


Materials Provided


  • P70204: 2 vials of 3 million stably transfected U2OS Cells expressing MCR3 and Nomad Biosensor
  • P70204-DA: 1 vial with 2 million Division Arrested U2OS Cells expressing MCR3 and Nomad Biosensor (READY TO USE)
 

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