This site uses third parties to own and optimize your navigation and perform analytical work cookies. If you continue browsing, consider that you accept its use.
You can learn more at our Cookie Policy.


  • Innoprot
    • Productos
      • GPCR Cell Lines
        • cAMP-NOMAD GPCR Cell Lines

PAC1R Nomad Cell Line

Reference: P70513

Get your Price

Data Sheet

A novel PAC1R Nomad cell line has been developed stably transfecting cAMP Nomad biosensor and human Pituitary  adenylate  cyclase‐activating polypeptide type 1 receptor (PAC1R) into U2OS cell line.

Innoprot cAMP Nomad PAC1R cell line has been designed to assay compounds or analyze their capability to modulate Pituitary  adenylate  cyclase‐activating polypeptide type 1 receptor. When the agonist binds to PAC1R a G protein is activated, which in turn, triggers a cellular response mediated by second messengers (cAMP).

Assay Details

In absence of cAMP, the fluorescent biosensor is localized in the cellular membrane. An increase in this second messenger concentration leads to change in the structural folding of Nomad Biosensor that promotes its cellular relocation in the vesicular trafficking of the cells. Activity is easily quantified on living cells by fluorescence intensity.

This highly reproducible assay has been validated using PACAP-38 as a PAC1R agonist in a High Throughput Screening (HTS) Platform. This cell line has been validated measuring cAMP increase in the cytosol analyzing both Nomad biosensor redistribution within the cell and intensity, showing an average of Z´= 0.80 +/- 0.01 for High Throughput Screening.

Materials Provided

  • P70513: 2 vials of 3 million stably transfected U2OS Cells expressing PAC receptor 1 and Nomad Biosensor
  • P70513-DA: 1 vial with 2 million Division Arrested U2OS Cells expressing PAC receptor 1 and Nomad Biosensor (READY TO USE)

Related Products/Services


© Innoprot 2008 - Cell Based Assays for Drug Discovery Screening - Cookie Policy