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Innoprot

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      • GPCR Cell Lines
        • Calcium-NOMAD GPCR Cell Lines

CCK2 Nomad Cell Line

Reference: P70108


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Data Sheet

A novel CCK2 Nomad cell line has been developed stably transfecting Ca++ Nomad biosensor and human Cholecystokinin B receptor (CCK2) into HEK293 cell line.

Innoprot Ca++ Nomad CCK2 cell line has been designed to assay compounds or analyze their capability to modulate Cholecystokinin B receptor. When the agonist binds to CCK2 a G protein is activated, which in turn, triggers a cellular response mediated by second messengers (Calcium).


Assay Details


In absence of calcium, the fluorescent biosensor is localized in the cellular membrane showing low fluorescence intensity. An increase in this second messenger concentration leads to change in the structural folding of Nomad Biosensor that promotes its cellular relocation showing an increase in fluorescence intensity. Activity is easily quantified on living cells by fluorescence intensity.

This highly reproducible assay has been validated using CCK Octapeptide, sulfated as a CCK2 agonist in a Multiplate Reader. This cell line has been validated measuring calcium increase in the cytosol analyzing green fluorescence intensity within the cell, showing an average of Z´= 0.79 +/- 0.01 for High Throughput Screening.



Materials Provided


  • P70108: 2 vials of 3 million stably transfected HEK293 Cells expressing CCK2 (CCKBR) receptor and Ca++ Nomad Biosensor.
 

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