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Alzheimer´s Disease Model - Tau Phosphorylation Assay

Data Sheet

The microtubule associated protein Tau is the main component of the neurofribillary tangles (NFT), aberrant structures that appear in the brain of Alzheimer´s disease patients and other tauopathies, such as FTDP-17 or corticobasal degeneration. Tau protein binds to and stabilizes microtubules (MTs) but in pathological states, it aggregates and loses its important functions. These Tau aggregates are composed basically by hyperphosphorylated and truncated forms of tau.

Multiple Tau gene mutations are pathogenic for hereditary FTDP-17 disease. These mutations have similar effects to hyperphosphorylation in Tau in AD and result in NFT formation.

Assay Details

U2OS stably expressing triple mutant human Tau-tGFP cells are treated with log dilution series of kinase inhibitors during 2-24 hours to evaluate their effects on Tau phosphorylation and binding to microtubules. Hyper phosporylation of Tau leads to its dissociation from microtubules and polymerization into tangles of paired helical filaments (PHF), characteristic of damaged brain tissues and one of the responsible for missfunction (loss of synaptic transmission) and neuronal cell death in tauopathies. On the contrary, kinase inhibitors promote Tau binding to microtubules and the formation of microtubule bundles. This bundle increase is detected and quantified by fluorescence using automated image analysis.



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