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Innoprot

Tumor cell migration & invasion Assay

Data Sheet

Tumor cell migration/invasion is one of the key steps in the tumor progression. The metastatic potential of tumor cells is largely dependent on their ability to degradate and migrate through extracellular matrix by proteolitic enzymes secretion.


Tumor Cell Migration Assay


Cell migration is performed using modified Boyden Chambers. Cells are seeded onto Collagen I, vitronectin or fibronectin-coated inserts with 8.0 μm pores and placed on top of 2-cm2 wells containing culture media. These proteins do not obstructe pores allowing cells moving through the pores. After a certain time, migrated cells are fixed, stained and counted using microscopy.


Tumor Cell Invasion Assay


Cell invasion is also performed using modified Boyden Chambers. Cells are seeded onto matrigel or gelatin-coated inserts with 8.0 μm pores and placed on top of 2-cm2 wells containing culture media. These proteins occlude 8μm pores and cells need an active enzymatic degradation for moving through the pores. 
After a certain time, cells are fixed, stained and counted using microscopy. Conditioned medium will be also collected and Metaloproteases (MMP-2 & MMP-9) will be measured by zimography.
 

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