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Mitochondrial Integrity Assay

Data Sheet

Mitochondria are cellular organelles regulating metabolism and cell death pathways. The membrane of the mitochondria is the key component to maintain the cell metabolism and homeostasis. Loss of membrane potential of mitochondria is the previous event of the cell dysfunction and is related directly with the activation of death pathways via caspase 3 activation.

A cell based assay is performed to measure the loss of mitochondrial   membrane potential using a fluorescent probe that can predict the cell dysfunction prior to complete cell collapse.

Assay Details

To evaluate the mitochondrial membrane potential (ΔΨm), the TMRM fluorescent dye is used. The tested compounds are incubated with the cells and following the TMRM dye is added and measured in the automated fluorescent image platform (Hamilton Robot and Pathway 855 BD). The fluorescent signal of tested compounds is compared with the control samples.

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