This site uses third parties to own and optimize your navigation and perform analytical work cookies. If you continue browsing, consider that you accept its use.
You can learn more at our Cookie Policy.


PD Model - Parkin Mitochondria Recruitment Assay

Data Sheet

Parkinson’s disease (PD) is a neurodegenerative disorder caused by the death of dopamine producing cells in the substantia nigra in the brain. Parkin is one of the proteins implicated in one form of familial PD known as autosomal recessive juvenile Parkinson's disease (AR-JP). Parkin ubiquitinates some proteins of the outer mitochondrial membrane, using this ubiquitination as an indication for both proteasome degradation and mitophagy.

Parkin is recruited to damaged mitochondria during the early stages of mitophagy induced by CCCP. PARK2-FP062+MTS-tGFP U2OS cell line is used to monitor parkin recruitment and screen compounds that inhibit mitochondrial loss of membrane potential.

Assay Details

To analyze PARK2 mitochondrial localization, a Region Of Interest (ROI) of PARK2 was delimited and the intensity of mitochondria signal (GFP) was quantified in this ROI. So, when PARK2 was recruited in the mitochondria by CCCP induction, the intensity of the GFP was increased. Compounds that inhibit mitochondrial loss of membrane potential will reduce the GFP increase induced by CCCP.



Request for Quotation

© Innoprot 2008 - Cell Based Assays for Drug Discovery Screening - Cookie Policy