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Innoprot

PD Model - Parkin Mitochondria Recruitment Assay

Data Sheet


Parkinson’s disease (PD) is a neurodegenerative disorder caused by the death of dopamine producing cells in the substantia nigra in the brain. Parkin is one of the proteins implicated in one form of familial PD known as autosomal recessive juvenile Parkinson's disease (AR-JP). Parkin ubiquitinates some proteins of the outer mitochondrial membrane, using this ubiquitination as an indication for both proteasome degradation and mitophagy.

Parkin is recruited to damaged mitochondria during the early stages of mitophagy induced by CCCP. PARK2-FP062+MTS-tGFP U2OS cell line is used to monitor parkin recruitment and screen compounds that inhibit mitochondrial loss of membrane potential.


Assay Details


To analyze PARK2 mitochondrial localization, a Region Of Interest (ROI) of PARK2 was delimited and the intensity of mitochondria signal (GFP) was quantified in this ROI. So, when PARK2 was recruited in the mitochondria by CCCP induction, the intensity of the GFP was increased. Compounds that inhibit mitochondrial loss of membrane potential will reduce the GFP increase induced by CCCP.


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