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Protein Interaction Service

Innoprot´s interaction service uses a affinity tag based-purification technology platform specially designed for high throughput analysis and identification of protein:protein & protein:rna interactions.

Defining protein & ribonucleoprotein complexes is critical to virtually all aspects of cell biology, because many cellular processes are regulated by stable protein complexes and their identification often provides insights into their function. Our technology platform can also be used to study disease relevant protein & ribonucleoprotein complexes in order to fully understand of the molecular mechanisms of diseases.

Our protein interaction service involves the characterization of protein & ribonucleoprotein complexes by expression of bait-proteins in mammalian cells. These baits are tools to isolate cellular proteins and their natural interactions partners as protein or ribonucleoprotein complex. They are isolated through affinity tag purification methods and subsequent identification of interaction partners through mass spectrometry, immunoprecipitation and Western blot analysis. After the isolation and identification of protein complexes, a functional analysis using RNA interference (RNAi) allow us to obtain more information about the cellular function of the protein complexes.


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