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Innoprot

  • Innoprot
    • Productos
      • Líneas Celulares GPCR
        • cAMP-NOMAD Cell Lines

GLP1R Nomad Cell Line

Referencia: P70203


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Ficha del Producto

A novel GLP1R Nomad cell line has been developed stably transfecting cAMP Nomad biosensor and glucagon-like peptide 1 receptor termed GLP1R into U2OS cell line.

Innoprot cAMP Nomad GLP1R cell line has been designed to assay compounds or analyze their capability to modulate glucagon-like peptide 1 receptor. When the agonist binds to GLP1R a G protein is activated, which in turn, triggers a cellular response mediated by second messengers (cAMP).


Assay Details


In absence of cAMP, the fluorescent biosensor is localized in the cellular membrane. An increase in this second messenger concentration leads to change in the structural folding of Nomad Biosensor that promotes its cellular relocation in the vesicular trafficking of the cells. Activity is easily quantified on living cells by fluorescence intensity but also by image analysis of cytoplasmatic granularity changes.

This highly reproducible assay has been validated using GLP-1 (7-37) as a GLP1R agonist in a microplate reader. This cell line has been validated measuring cAMP increase in the cytosol analyzing both fluorescence intensity and Nomad biosensor distribution within the cell, showing an average of Z´= 0.80 +/- 0.01.


Materials Provided


  • P70203: 2 vials of 3 million stably transfected U2OS Cells expressing GLP1R and Nomad Biosensor
   

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