Immortalized Human Osteoblasts - Image 2

Immortalized Human Osteoblasts

Reference: P10979-IM

2.085,00€

Immortalized Human Osteoblasts (IM-HO) provided by Innoprot have been developed by immortalizing primary human osteoblast with SV40 Large T antigen.

Immortalized cells were controlled passaging side by side with the primary cells. Primary cells go into senescence after the 5th passage while the SV40‐tranduced cells go beyond 30 passages.

Each vial of Immortalized Human Osteoblasts contains more than 1 million viable cells. Innoprot also offers optimized medium and reagents for the growth of IM-HO which are quality tested together and guaranteed to give maximum performance as a global solution for in vitro IM-HO culture.

Size/Quantity: 1 million cells / vial

Immortalized Human Osteoblasts have been obtained from human primary healthy osteoblasts. Bone is a dynamic tissue, whose remodeling occurs continuously through the coordinated actions of both, osteoclasts and osteoblasts. Osteoblasts, the bone-forming cells, come from originally from pluripotent mesenchymal stem cells. They synthesize and secrete organic extracellular matrix, osteoid, whose main component is type I collagen. Osteoid is calcified by osteoblasts and during this process the cells become encased in lacunae within the calcified material and become osteocytes. Human Osteoblasts express protease-activated receptor-1 and vascular endothelial cell growth factor. Leukemia inhibitory factor can bind to the osteoblast cell surface and induce bone formation both in vitro and in vivo. The balance between osteoblast recruitment, proliferation, differentiation and apoptosis in sutures between cranial bones is essential for calvarial bone formation. Immortalized Human Osteoblasts are also useful tools to stablish in vitro disease models for High Throughput and High Content Screening.