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Vasopressin Receptor 2 Cell Line

cAMPNOMAD Vasopressin Receptor 2 Cell Line

12.500,00

cAMPNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and cAMP Nomad Biosensor to monitor G-protein modulation by cAMP release. Each vial of cAMPNOMAD Vasopressin Receptor 2 Cell Line contains U2OS cells stably expressing the following constructs:

  • human Vasopressin Receptor 2 (AVPR2) with no tag
  • cAMP Nomad Biosensor (Red Fluorescence)

 

cAMPNOMAD AVPR2 Receptor Cell Line allows to assay compounds analyzing the G-Protein signalling pathway by Gs involving receptor activation. When an agonist binds to [Arg8]‐Vasopressin, the Gs protein is activated which, in turn, triggers a cellular response mediated by cAMP via adenylate cyclase stimulation. This leads to an increase of red fluorescence intensity of cAMP biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.

 

Size/Quantity: 2 vials containing 3×106 cells / vial

NOMAD VPAC2 Receptor Cell Line

cAMPNOMAD VPAC2 Receptor Cell Line

12.500,00

cAMPNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and cAMP Nomad Biosensor to monitor G-protein modulation by cAMP release. Each vial of cAMPNOMAD VPAC2 Receptor Cell Line contains U2OS cells stably expressing the following constructs:

  • human VIP Receptor 2 (VPAC2R) with no tag
  • cAMP Nomad Biosensor (Red Fluorescence)

cAMPNOMAD VPAC2 Receptor Cell Line allows to assay compounds analyzing the G-Protein signalling pathway by Gs involving receptor activation. When an agonist binds to PACAP-38, the Gs protein is activated which, in turn, triggers a cellular response mediated by cAMP via adenylate cyclase stimulation. This leads to an increase of red fluorescence intensity of cAMP biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.

Size/Quantity: 2 vials containing 3×106 cells / vial

NOMAD VPAC1 Receptor Cell Line

cAMPNOMAD VPAC1 Receptor Cell Line

12.500,00

cAMPNOMAD Cells are cell lines stably co-expressing tag-free GPCRs and cAMP Nomad Biosensor to monitor G-protein modulation by cAMP release. Each vial of cAMPNOMAD VPAC1 Receptor Cell Line contains U2OS cells stably expressing the following constructs:

  • human VIP Receptor 1 (VPAC1R) with no tag
  • cAMP Nomad Biosensor (Red Fluorescence)

cAMPNOMAD VPAC1 Receptor Cell Line allows to assay compounds analyzing the G-Protein signalling pathway by Gs involving receptor activation. When an agonist binds to PACAP-38, the Gs protein is activated which, in turn, triggers a cellular response mediated by cAMP via adenylate cyclase stimulation. This leads to an increase of red fluorescence intensity of cAMP biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.

Size/Quantity: 2 vials containing 3×106 cells / vial

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