5-HT2C Serotonin Receptor Assay (HTR2C) from Innoprot allows to assay compounds, or analyze their capability to modulate HTR2C. When a ligand binds to the HTR2C, it activates a G protein; which in turn, triggers a cellular response mediated by calcium. The binding of an agonist to HTR2C induces mobilization of intracellular calcium and also activation of PKC via the PLC–IP3 and DAG pathways, respectively.
5-HT2C Serotonin Receptor Assay comprises the measurement of calcium concentration in the cytosol. The high reproducibility of this assay allows monitoring HTR2C receptor activation process in living cells.
Cell line used: HiTSeeker 5HTR2C Cell Line
Readout: Calcium Flux
EC50 Agonist: 1.16 x 10-9 M for Calcium Flux
Type of Assay: Cell-based / Functional
Detection Method: Fluorimetry