alpha1A Adrenoceptor assay (ADRA1A) from Innoprot provides the analysis of both signalling pathways involving receptor activation; G protein by Calcium flux and β-arrestin recruitment using Nomad multiplex technology.
The alpha-1A adrenergic receptor (α1A adrenoreceptor), also known as ADRA1A, is member of Alpha-1-adrenergic receptors (alpha-1-ARs) family. They activate mitogenic responses and regulate growth and proliferation of many cells. The primary transduction mechanism is mediated by Gq/G11 family transducers, so its activation response is easily measured by calcium flux.
Cell line used: ADRA1A Nomad Cell Line
Readout: Calcium Flux & β-arrestin recruitment
Agonist: A61603
EC50 Agonist: 1.26 x 10-11 M for Calcium Assay & 5.97 x 10-11 for β-arrestin recruitment
Type of Assay: Cell-based / Functional
Detection Method: Fluorimetry
Assay: Adrenergic Receptors, Cardiovascular, CNS, GPCR, Parkinson's Disease
Area: Cardiovascular, CNS, Parkinson's Disease