alpha1A adrenoreceptor assay

alpha1A Adrenoceptor assay (ADRA1A) from Innoprot provides the analysis of both signalling pathways involving receptor activation; G protein by Calcium flux and β-arrestin recruitment using Nomad multiplex technology.

The alpha-1A adrenergic receptor (α1A adrenoreceptor), also known as ADRA1A, is member of Alpha-1-adrenergic receptors (alpha-1-ARs) family. They activate mitogenic responses and regulate growth and proliferation of many cells. The primary transduction mechanism is mediated by Gq/G11 family transducers, so its activation response is easily measured by calcium flux.

Cell line used: ADRA1A Nomad Cell Line

Readout: Calcium Flux & β-arrestin recruitment

Agonist: A61603

EC50 Agonist: 1.26 x 10-11 M for Calcium Assay & 5.97 x 10-11 for β-arrestin recruitment

Type of Assay: Cell-based / Functional

Detection Method: Fluorimetry

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