APP Processing Assay

Alzheimer’s Disease (AD) comprises the accumulation of brain depositions of the beta amyloid (βA). The βA is the amyloid precursor protein (APP) digestion product, released from the cell after secretases proteolysis.This is the basis of the APP Processing Assay offered by Innoprot.

Innoprot has developed a novel fluorescence-based assay for screening of APP Processing Assayinhibitors of the APP Processing pathway. This novel assay requires the use of a MDCK cell line stably expressing green fluorescent APP. Treating this cell line with Aβ peptide formation inhibitors such as secretase activity inhibitors, APP appears retained in fluorescent spots. It occurs in a dose-response manner, allowing the quantification with imaging systems.

This model identifies β-secretase and γ-secretase inhibitors, but also any other compound which stops Aβ peptide formation.


Cell line used: tGFP-APP MDCK

Readout: Fluorescent tGFP-APP retained vesicles

Type of Assay: Cell-based / Functional

Detection Method: Image Analysis

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