Cardiac Fibrosis Assay from Innoprot enables monitoring TGF-β/Smad signaling activation on Human Cardiac Fibroblasts.
Immortalized primary Human Cardiac Fibroblasts stimulated with TGF-β1 demonstrate a clear concentration-dependent increase in Smad Binding Elements (SBE) expression; while increasing the TGF-β1 stimuli showes no effect of on the number of nuclei, indicative of no cytotoxic events. TGF-β trigger is inhibited by treatment with the ALK-5 inhibitor, SB525334, showing full inhibition of SBE expression regardless of the presence of TGF-β1.
Cell line used: Immortalized primary Human Cardiac Fibroblasts
Readout: Smad activation
Type of assay: Cell-based
Fibrosis Induction: TGF-β
Reference inhibitor: SB525334