CCR2 Chemokine Receptor Internalization Assay from Innoprot allows to assay compounds, or analyze their capability to modulate CCR2 receptor activation and the following redistribution process inside the cells. When a ligand binds to the CCR2, it activates a G protein, which internalizes in big and high intensity vesicles.
This assay has been validated measuring Fluorescent CCR2 redistribution by increase of fluorescence surrounding the nuclei using image analysis algorithms. The high reproducibility of this assay allows monitoring CCR2 Chemokine Receptor redistribution process in High Throughput Screening.
Cell line used: Green Fluorescent CCR2 Chemokine Receptor Cell Line
Readout: Fluorescent Receptor Internalization
EC50 Agonist: 0.73 ng/ml
Type of Assay: Cell-based
Detection Method: Image Analysis