D5 Dopamine Receptor Assay from Innoprot allows the measurement of DRD5 receptor activation by cAMP increase in cell cytosol. We assay test-compounds analyzing the G-Protein signalling pathway by Gs involving DRD5 receptor activation. When an agonist binds to DRD5, it activates the Gs protein which; in turn, triggers a cellular response mediated by cAMP via adenylate cyclase stimulation. This leads to an increase of fluorescence intensity of cAMP Nomad biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.
Cell line used: DRD5 Nomad Cell Line
Readout: cAMP Flux (Gs activation)
EC50 Agonist: 1.66 x 10-6 M for cAMP Flux
Type of Assay: Cell-based / Functional
Detection Method: Fluorimetry