Gamma secretase activity assay from Innoprot allows the monitoring of γ-secretase proteolytic process in living cells. Assay is performed on a cell line expressing a fluorescent construct (APP-C99) containing the γ-secretase cleaving site. When γ-secretase activity is inhibited by any antagonist, fluorescent aggregates appear in the cell cytoplasm in a dose-response manner.
Cell line used: APP-C99-tGFP U2OS
Readout: Fluorescent APP-C99 aggregates quantification
Antagonist: DAPT
EC50 Antagonist: 1.10 x 10-7 M
Type of Assay: Cell-based / Functional
Detection Method: Image Analysis