Gamma secretase activity assay

Gamma secretase activity assay from Innoprot allows the monitoring of γ-secretase proteolytic process in living cells. Assay is performed on a cell line expressing a fluorescent construct (APP-C99) containing the γ-secretase cleaving site. When γ-secretase activity is inhibited by any antagonist, fluorescent aggregates appear in the cell cytoplasm in a dose-response manner.

gamma secretase activity assay

Cell line used: APP-C99-tGFP U2OS

Readout: Fluorescent APP-C99 aggregates quantification

Antagonist: DAPT

EC50 Antagonist: 1.10 x 10-7 M

Type of Assay: Cell-based / Functional

Detection Method: Image Analysis

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