GLP-2 Glucagon Receptor Assay from Innoprot measures GLP2R receptor activation using Nomad biosensors technology. GLP-2 Glucagon Receptor signaling occurs via adenylate cyclase stimulation through trimeric Gs proteins, increasing cAMP, and downstream L-type calcium channel interaction. cAMP-Nomad Biosensor measures the receptor activation by cAMP accumulation. The high reproducibility of this assay allows monitoring GLP-2 Glucagon Receptor activation process in High Throughput Screening.

GLP-2 Glucagon Receptor is a receptor for glucagon-like peptide 2. GLP2 is a 33-amino acid proglucagon peptide subproduct and the intestinal enteroendocrine cells release it. GLP2 stimulates intestinal growth and upregulates villus height in the small intestine. Moreover, GLP2 prevents intestinal hypoplasia resulting from total parenteral nutrition. GLP2R is present in the gut and in enteroendocrine cells in the intestinal tract.

Cell line used: GLP2R Nomad Cell Line

Readout: cAMP Flux (Gs activation)

Agonist: GLP-2

EC50 Agonist: 3.44 x 10-8 M for cAMP Flux

Type of Assay: Cell-based / Functional

Detection Method: Fluorimetry

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