Neurotensin Receptor 1 Assay

Human Neurotensin receptor 1 assay (NTSR1) from Innoprot provides the analysis of both signalling pathways involving GPCR activation; G protein by Calcium flux and β-arrestin recruitment using Nomad multiplex technology. The primary transduction mechanism involves Gq/G11 family transducers, so we measure Ca2+ flux in combination with β-arrestin recruitment in this assay.

Neurotensin receptor 1 belongs to the large superfamily of G-protein coupled receptors. NTSR1 mediates the multiple functions of neurotensin, such as hypotension, hyperglycemia, hypothermia, antinociception, and regulation of intestinal motility and secretion.

Receptor Information: NTS1 Receptor


Cell line used: NTSR1 Nomad Cell Line

Readout: Calcium Flux & β-arrestin recruitment

Agonist: Neurotensin

EC50 Agonist: 3.38 x 10-9 M for Calcium Assay & 7.79 x 10-9 for β-arrestin recruitment

Antagonist: SR48692

EC50 Antagonist: 1.08 x 10-7 M for Calcium Assay & 1.42 x 10-7 for β-arrestin recruitment

Type of Assay: Cell-based / Functional

Detection Method: Fluorimetry

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