Human Neurotensin receptor 1 assay (NTSR1) from Innoprot provides the analysis of both signalling pathways involving GPCR activation; G protein by Calcium flux and β-arrestin recruitment using Nomad multiplex technology. The primary transduction mechanism involves Gq/G11 family transducers, so we measure Ca2+ flux in combination with β-arrestin recruitment in this assay.
Neurotensin receptor 1 belongs to the large superfamily of G-protein coupled receptors. NTSR1 mediates the multiple functions of neurotensin, such as hypotension, hyperglycemia, hypothermia, antinociception, and regulation of intestinal motility and secretion.
Receptor Information: NTS1 Receptor
Cell line used: NTSR1 Nomad Cell Line
Readout: Calcium Flux & β-arrestin recruitment
Agonist: Neurotensin
EC50 Agonist: 3.38 x 10-9 M for Calcium Assay & 7.79 x 10-9 for β-arrestin recruitment
Antagonist: SR48692
EC50 Antagonist: 1.08 x 10-7 M for Calcium Assay & 1.42 x 10-7 for β-arrestin recruitment
Type of Assay: Cell-based / Functional
Detection Method: Fluorimetry
Assay: ALS, Cardiovascular, CNS, GPCR, Neurotensin receptors, Oncology, Pain
Area: Cardiovascular, CNS, Inflammation, Pain