NK3 Tachykinin Receptor Assay (TACR3) from Innoprot allows to assay compounds, or analyze their capability to modulate TACR3. When a ligand binds to the TACR3, it activates a G protein; which in turn, triggers a cellular response mediated by calcium. The binding of an agonist to TACR3 induces mobilization of intracellular calcium and also activation of PKC via the PLC–IP3 and DAG pathways, respectively.
NK3 Tachykinin Receptor Assay comprises the measurement of calcium concentration in the cytosol. The high reproducibility of this assay allows monitoring TACR3 receptor activation process in living cells.
Cell line used: HiTSeeker TACR3 Cell Line
Readout: Calcium Flux
Agonist: Substance P
EC50 Agonist: 5.30 x 10-8 M for Calcium Flux
Type of Assay: Cell-based / Functional
Detection Method: Fluorimetry