PAC1 Receptor Assay

PAC1 Receptor Assay from Innoprot measures PAC1 receptor activation using HTRF technology. PAC1 Receptor signaling occurs via adenylate cyclase stimulation through trimeric Gs proteins, increasing cAMP, and downstream L-type calcium channel interaction.

In this assay, we measure the receptor activation/inhibition by cAMP accumulation using HTRF technology. The high reproducibility of this assay allows monitoring PAC1 Receptor activation process in living cells.

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Cell line used: HiTSeeker PAC1R Cell Line

Readout: cAMP Flux (HTRF)

Agonist: PACAP-38

EC50 Agonist: 5.13 x 10-10 M for cAMP Flux

Type of Assay: Cell-based / Functional

Detection Method: Fluorimetry

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