PAR2 Functional Assay

Proteinase-activated Receptor 2 – PAR2 Receptor Assay from Innoprot provides the analysis of both signalling pathways involving GPCR activation; G protein by Calcium flux and β-arrestin recruitment using Nomad multiplex technology. The primary transduction mechanism involves Gq/G11 family transducers, so we measure Ca2+ flux in combination with β-arrestin recruitment in this assay.

Protease activated receptor 2 (PAR2) is member of the protease-activated receptor family. Other names for this receptor are coagulation factor II (thrombin) receptor-like 1 (F2RL1) and G-protein coupled receptor 11 (GPR11). PAR2 modulates inflammatory responses, obesity, metabolism, and acts as a sensor for proteolytic enzymes generated during infection.

Receptor Information: PAR2 Receptor

Cell line used: PAR2 Nomad Cell Line

Readout: Calcium Flux & β-arrestin recruitment

Agonist: SLIGKV

EC50 Agonist: 1.67 x 10-6 M for Calcium Assay & 4.83 x 10-6 for β-arrestin recruitment

Type of Assay: Cell-based / Functional

Detection Method: Fluorimetry

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