M4 Muscarinic Acetylcholine Receptor Assay (CHRM4) from Innoprot measures CHRM4 receptor activation using Nomad Biosensors technology. CHRM4 signaling occurs via adenylate cyclase inhibition through trimeric Gi/o proteins, reducing cAMP via PKA activation. In this assay, we measure the receptor activation/inhibition by PKA activation using Nomad Biosensors technology. The high reproducibility of this assay allows monitoring…
M3 Muscarinic Acetylcholine Receptor Assay (CHRM3) from Innoprot allows to assay compounds, or analyze their capability to modulate CHRM3. When a ligand binds to the CHRM3, it activates a G protein; which in turn, triggers a cellular response mediated by calcium. The binding of an agonist to CHRM3 induces mobilization of intracellular calcium and also…
M1 Muscarinic Acetylcholine Receptor Assay (CHRM1) from Innoprot allows to assay compounds, or analyze their capability to modulate CHRM1. When a ligand binds to the CHRM1, it activates a G protein; which in turn, triggers a cellular response mediated by calcium. The binding of an agonist to CHRM1 induces mobilization of intracellular calcium and also…
S1P3 Lysophospholipid Receptor Assay (S1P3R) from Innoprot provides the analysis of both signalling pathways involving S1P3R activation; G Protein by the analysis of Gi/Go activation and also β-arrestin recruitment using Nomad multiplex technology. When an agonist binds to S1P3R, it activates Gi/Go, which in turn, triggers a cellular response involving PKA activation. In this assay…
MC3 Melanocortin Receptor Assay from Innoprot measures MC3R receptor activation using Nomad Biosensors technology. MC3 Melanocortin Receptor signaling occurs via adenylate cyclase stimulation through trimeric Gs proteins, increasing cAMP, and downstream L-type calcium channel interaction. In this assay, we measure the receptor activation/inhibition by cAMP accumulation using cAMP-Nomad Biosensor. The high reproducibility of this assay…
MC4 Melanocortin Receptor Assay from Innoprot measures MC4R receptor activation using HTRF technology. MC4 Melanocortin Receptor signaling occurs via adenylate cyclase stimulation through trimeric Gs proteins, increasing cAMP, and downstream L-type calcium channel interaction. In this assay, we measure the receptor activation/inhibition by cAMP accumulation using HTRF technology. The high reproducibility of this assay allows…
S1P1 Lysophospholipid Receptor Internalization Assay from Innoprot allows to assay compounds, or analyze their capability to modulate S1P1 receptor activation and the following redistribution process inside the cells. When a ligand binds to the S1P1R, it activates a G protein, which internalizes in big and high intensity vesicles. This assay has been validated measuring Fluorescent…
LPA3 Lysophospholipid Receptor Assay from Innoprot allows to assay compounds, or analyze their capability to modulate LPAR3. When a ligand binds to the LPAR3, it activates a G protein, which in turn, triggers a cellular response comprising Calcium. Our LPAR3 assay measures Calcium concentration changes in the cytosol using LPA as positive control. The high…
GLP-2 Glucagon Receptor Assay from Innoprot measures GLP2R receptor activation using Nomad biosensors technology. GLP-2 Glucagon Receptor signaling occurs via adenylate cyclase stimulation through trimeric Gs proteins, increasing cAMP, and downstream L-type calcium channel interaction. cAMP-Nomad Biosensor measures the receptor activation by cAMP accumulation. The high reproducibility of this assay allows monitoring GLP-2 Glucagon Receptor…