Innoprot offers phenotypic assays to better understand the complex interplay of networks and pathways analyzing different cellular processes. The development of these cell-based assays comprises primary cells, but also recombinant cell lines mimmicking disease pathways.
At Innoprot we have also developed complex in vitro disease models for different diseases; such as Alzheimer’s Disease, Parkinson’s Disease, Amyotrophic Lateral Sclerosis (ALS) and Fibrosis.
About 10% of ALS cases are inherited; and a large subset of them are caused by mutations in the gene encoding the copper-zinc superoxide dismutase (SOD1). The detection of SOD1-positive inclusions in familial ALS patients suggests the role of SOD1 aggregation underlying the pathology of familial ALS. Although SOD1 mutant proteins are different in structure,…
α-Synuclein is a presynaptic neuronal protein which may contribute genetically and neuropathologically to Parkinson’s disease (PD). Aggregated a-synuclein is the major component of inclusions in Parkinson’s disease; but also in other synucleinopathy brains indicating that α-synuclein aggregation may play a main role in the pathogenesis of neurodegenerative disorders. In this α-synuclein aggregation assay from Innoprot,…
Our Astrocytosis assay enables the study of fibrosis-related parameters on TGF-β1 induced Human primary astrocytes. Astrocytosis assay from Innoprot allows the evaluation of therapeutic compounds in this disease area by measuring several biomarkers; including Fibronectin, ASMA and also Collagen via high content analysis. Astrocytosis is a common neurocellular manifestation of brain pathology in individuals with…
Cardiac Fibrosis Assay from Innoprot enables monitoring TGF-β/Smad signaling activation on Human Cardiac Fibroblasts. Immortalized primary Human Cardiac Fibroblasts stimulated with TGF-β1 demonstrate a clear concentration-dependent increase in Smad Binding Elements (SBE) expression; while increasing the TGF-β1 stimuli showes no effect of on the number of nuclei, indicative of no cytotoxic events. TGF-β trigger is…
Renal Fibrosis Assay from Innoprot enables the study of fibrosis-related parameters on TGF-β induced Renal Tubular Epithelial Cells. Human Renal Tubular Epithelial Cells stimulated with TGF-β1 demonstrate a clear concentration-dependent increase in αSMA levels; while increasing the TGF-β1 stimuli showes no effect of on the number of nuclei, indicative of no cytotoxic events. TGF-β trigger…
Endothelial Cell Tube Formation Assay from Innoprot allows to analyze the effect of test-compounds to induce/inhibit new blood vessels formation. We determine the IC50 values of your compounds of interest in whole cell assays using different organ-specific endothelial cells; such as liver, renal, retinal, colonic, lung, pancreas or umbilical endothelial cells. We offer over 20…
Tumor Cell Proliferation Assay from Innoprot includes allows to analyze the effect of test-compounds to increase/decrease cell proliferation. We determine the IC50 values of your compounds of interest in whole cell assays using different techniques; such as MTT, LDH release, CellTiter or nuclei quantification. We offer over 60 different tumor cell lines for cell screening…
Tumor Cell Adhesion Assay from Innoprot allows to analyze the effect of drugs that could affect the physiological interaction between tumor and stromal cells. Besides this assay is very useful to identify adhesion molecules involving in the process of interaction with stromal cells or extracellular matrix substrates. Therefore, an in vitro cell co-culture system for…
Several processes or insults can induce damage on DNA; such as a cell death pathway activation or by the genotoxic activity of a compound; substances that makes them harmful to the genetic information contained in organisms. Genotoxins are the substances that have the property of genotoxicity. All new chemical entities in the drug discovery process…