Astrocytosis Assay

Astrocytosis Assay

Our Astrocytosis assay enables the study of fibrosis-related parameters on TGF-β1 induced Human primary astrocytes. Astrocytosis assay from Innoprot  allows the evaluation of therapeutic compounds in this disease area by measuring several biomarkers; including Fibronectin, ASMA and also Collagen via high content analysis. Astrocytosis is a common neurocellular manifestation of brain pathology in individuals with…

Cardiac Fibrosis Assay

Cardiac Fibrosis Assay

Cardiac Fibrosis Assay from Innoprot enables monitoring TGF-β/Smad signaling activation on Human Cardiac Fibroblasts. Immortalized primary Human Cardiac Fibroblasts stimulated with TGF-β1 demonstrate a clear concentration-dependent increase in Smad Binding Elements (SBE) expression; while increasing the TGF-β1 stimuli showes no effect of on the number of nuclei, indicative of no cytotoxic events. TGF-β trigger is…

Renal Fibrosis Assay

Renal Fibrosis Assay

Renal Fibrosis Assay from Innoprot enables the study of fibrosis-related parameters on TGF-β induced Renal Tubular Epithelial Cells. Human Renal Tubular Epithelial Cells stimulated with TGF-β1 demonstrate a clear concentration-dependent increase in αSMA levels; while increasing the TGF-β1 stimuli showes no effect of on the number of nuclei, indicative of no cytotoxic events. TGF-β trigger…

Aβ1-40 Induced Neurotoxicity Assay

Aβ1-40 Induced Neurotoxicity Assay

Intoxication of neurons with beta-amyloid (1-40) is a useful Alzheimer disease (AD) in vitro model. This model allows evaluate the capacity of different agents to inhibit the neuronal damage in AD based on the accumulation of neurotoxic amyloid beta peptide. Aβ1-40 Induced Neurotoxicity Assay offers the advantage of a rapid screening of the neuroprotective potential…

Aβ1-42 Induced Neurotoxicity Assay

Aβ1-42 Induced Neurotoxicity Assay

Intoxication of neurons with beta-amyloid (1-42) oligomers is a useful Alzheimer disease (AD) in vitro model. This model allows evaluate the capacity of different agents to inhibit the neuronal damage in AD based on the accumulation of neurotoxic amyloid beta oligomers. Aβ1-42 Induced Neurotoxicity Assay offers the advantage of a rapid screening of the neuroprotective…

Ischemic Stroke in vitro Assay

Ischemic Stroke in vitro Assay

Ischemic Stroke in vitro assay is based on the exposure of rat primary neurons under Oxygen-Glucose Deprivation conditions. Ischemia-like conditions are induced by replacing the normal O2/CO2 equilibrated medium with 94,7% N2 /5% CO2/0,3% O2 medium in a hypoxic chamber. We also use a culture medium without glucose to expose the cells to real OGD…

Amyloid-beta peptide Oligomerization Assay

Amyloid-beta peptide Oligomerization Assay

Amyloid-beta peptide Oligomerization Assay is a functional cell-based assay to identify inhibitors of amyloid peptide oligomerization process. We incubate rat primary neurons, human Microglia or SH-SY5Y cells with test compounds in parallel with Aβ1-42 peptide during 24h. After that, we measure Aβ1-42 oligomerization process analysing the number of thiazine staining oligomers related to cell number .

APP Processing Assay

APP Processing Assay

Alzheimer’s Disease (AD) comprises the accumulation of brain depositions of the beta amyloid (βA). The βA is the amyloid precursor protein (APP) digestion product, released from the cell after secretases proteolysis.This is the basis of the APP Processing Assay offered by Innoprot. Innoprot has developed a novel fluorescence-based assay for screening of inhibitors of the…

Parkin Mitochondrial Recruitment Assay

Parkin Mitochondrial Recruitment Assay

Parkin mitochondrial recruitment assay from Innoprot is based of localization changes of two fluorescent proteins; the Parkin fluorescent fusion polypeptide that changes its localization from the cytosol to the outer mitochondrial membrane (OMM); and the other fluorescent fusion polypeptide (MTS-tGFP), which binds directly to OMM, allowing the mitochondrial integrity analysis. Several compounds promote Parkin recruitment,…